Journal: Emerging Microbes & Infections
Article Title: Potent RBD-specific neutralizing rabbit monoclonal antibodies recognize emerging SARS-CoV-2 variants elicited by DNA prime-protein boost vaccination
doi: 10.1080/22221751.2021.1942227
Figure Lengend Snippet: Functional screening of cultured S1-specific single rabbit B cell clones. (A) The sorting strategy for S1-specific B cells from rabbit splenocytes using FACS. (B) High-throughput functional screening of cultured B cells. The number of individually cultured B cell clones (Cultured B cell clones), B cell clones binding SARS-CoV-2 S1 protein (SARS-CoV-2 S1 binding clones), B cell clones binding the SARS-CoV-2 RBD (SARS-CoV-2 RBD binding clones), B cell clones with neutralization potential (Neutralization clones), and B cell clones with the capability of inhibiting RBD and ACE2 interaction (RBD/ACE2 blocking clones) were listed. (C) Representative ELISA screening of cultural supernatants for top 40 B cell clones that can bind to the SARS-CoV-2 S1 protein. (D) Pseudovirus neutralization assay of S1-specific B cell cultural supernatants. (E) ACE2 blocking assay of S1-specific B cell cultural supernatants.
Article Snippet: Recombinant ACE2 (Kactus Biosystems, Cat. No. ACE-HM501) was coated at 1 μg/ml on an ELISA plate.
Techniques: Functional Assay, Cell Culture, Clone Assay, High Throughput Screening Assay, Binding Assay, Neutralization, Blocking Assay, Enzyme-linked Immunosorbent Assay